Undergraduate Degree: BS
Undergraduate Degree Year: 1997
Undergraduate Institution: Brigham Young University
Undergraduate Major: Biochemistry
PhD Degree Institution: Brigham Young University
PhD Degree Date: April, 2002
Year in Program: A
Graduate Program: Molecular Virology & Microbiology
Research Advisor First Name: Ata Murat
Research Advisor Last Name: Kaynar
Research Title/Research Topic: Therapeutic benefit of a prophylactic administration of OA-NO2 in protecting from hyperoxia-induced lung injury
Current Research Description: Tuberculosis (TB) is a significant global health problem where it is estimated that approximately one-third of the world’s population is infected causing ~1.9 million deaths per year. Although effective chemotherapy is available for most patients, the long duration of daily treatment is difficult to administer among the disadvantaged and in the developing world. Current therapy requires daily dosing over the course of six months or greater. The scientific reasons behind the need for such an extended treatment time are not clear. To address this our laboratory is pioneering a new imaging approach using PET/CT. One of the major limitations in studying TB chemotherapies has been the inability to interrogate and track changes in the microenvironment of individual lesions. Coupling PET with CT has made it possible for us to discriminate anatomically similar lesions based upon their metabolic status, functional characteristics and response to anti-TB therapies. This approach allows us for the first time to be able to follow and directly evaluate the efficacy of TB chemotherapy at a lesion-specific level.
Student Committee Participation
Student Committee Involvement:
Grant Support Information:
PEER REVIEWED PUBLICATIONS
1. Kesav, S., McLaughlin, J. & Scallen, T. J. (1992) Participation of sterol carrier protein-2 in cholesterol metabolism, Biochem Soc Trans. 20, 818-24.
2. Gaudet, R., Savage, J. R., McLaughlin, J. N., Willardson, B. M. & Sigler, P. B. (1999) A molecular mechanism for the phosphorylation-dependent regulation of heterotrimeric G proteins by phosducin, Mol Cell. 3, 649-60.
3. Savage, J. R., McLaughlin, J. N., Skiba, N. P., Hamm, H. E. & Willardson, B. M. (2000) Functional roles of the two domains of phosducin and phosducin-like protein, J Biol Chem. 275, 30399-407.
4. Thulin, C. D., Savage, J. R., McLaughlin, J. N., Truscott, S. M., Old, W. M., Ahn, N. G., Resing, K. A., Hamm, H. E., Bitensky, M. W. & Willardson, B. M. (2001) Modulation of the G protein regulator phosducin by Ca2+/calmodulin-dependent protein kinase II phosphorylation and 14-3-3 protein binding, J Biol Chem. 276, 23805-15.
5. Martin, M. M., Willardson, B. M., Burton, G. F., White, C. R., McLaughlin, J. N., Bray, S. M., Ogilvie, J. W., Jr. & Elton, T. S. (2001) Human angiotensin II type 1 receptor isoforms encoded by messenger RNA splice variants are functionally distinct, Mol Endocrinol. 15, 281-93.
6. McLaughlin, J. N., Thulin, C. D., Bray, S. M., Martin, M. M., Elton, T. S. & Willardson, B. M. (2002) Regulation of angiotensin II-induced G protein signaling by phosducin-like protein, J Biol Chem. 277, 34885-95.
7. McLaughlin, J. N., Thulin, C. D., Hart, S. J., Resing, K. A., Ahn, N. G. & Willardson, B. M. (2002) Regulatory interaction of phosducin-like protein with the cytosolic chaperonin complex, Proc Natl Acad Sci U S A. 99, 7962-7.
8. Martin-Benito, J., Bertrand, S., Hu, T., Ludtke, P. J., McLaughlin, J. N., Willardson, B. M., Carrascosa, J. L. & Valpuesta, J. M. (2004) Structure of the complex between the cytosolic chaperonin CCT and phosducin-like protein, Proc Natl Acad Sci U S A. 101, 17410-5.
9. McLaughlin, J. N., Mazzoni, M. R., Cleator, J. H., Earls, L., Perdigoto, A. L., Brooks, J. D., Muldowney, J. A., 3rd, Vaughan, D. E. & Hamm, H. E. (2005) Thrombin modulates the expression of a set of genes including thrombospondin-1 in human microvascular endothelial cells, J Biol Chem. 280, 22172-80. Epub 2005 Apr 7.
10. Lukov, G. L., Hu, T., McLaughlin, J. N., Hamm, H. E. & Willardson, B. M. (2005) Phosducin-like protein acts as a molecular chaperone for G protein betagamma dimer assembly, Embo J. 24, 1965-75. Epub 2005 May 5.
11. McLaughlin, J. N., Shen, L., Holinstat, M., Brooks, J. D., Dibenedetto, E. & Hamm, H. E. (2005) Functional selectivity of G protein signaling by agonist peptides and thrombin for the protease-activated receptor-1, J Biol Chem. 280, 25048-59.
12. Holinstat, M., Voss, B., Bilodeau, M. L., McLaughlin, J. N., Cleator, J. & Hamm, H. E. (2006) PAR4, but not PAR1, signals human platelet aggregation via Ca2+ mobilization and synergistic P2Y12 receptor activation, J Biol Chem. 281, 26665-74.
13. Voss, B., McLaughlin, J. N., Holinstat, M., Zent, R. & Hamm, H. E. (2007) PAR1, but not PAR4, activates human platelets through a Gi/o/PI3K signaling axis, Mol Pharmacol, mol.106.033365.
14. McLaughlin, J. N., Patterson, M. M. & Malik, A. B. (2007) Protease-activated receptor-3 (PAR3) regulates PAR1 signaling by receptor dimerization, Proc Natl Acad Sci U S A. 104, 5662-7.
15. McLaughlin, J. N., Ramachandran, R., Kaynar, A. M., Shapiro, S. D., Angus, D. C. & Malik, A. B. (2010) Protease-activated receptor-1 (PAR-1) dimerization with endothelial protein C receptor (EPCR) redirects heterotrimeric G-protein signaling to an endothelial protective/anti-inflammatory phenotype, manuscript in submission.
NON-PEER REVIEWED PUBLICATIONS
1. JN McLaughlin, R Ramachandran, AM Kaynar, SD Shapiro, DC Angus and AB Malik, Regulation of endothelial function by coagulation proteases in sepsis, Critical Care 2011, 15(Suppl 1):P251.
2. J McLaughlin, J Rella, A Bakan, L Kong, L Zhu, D Frederick, S Yende, R Ferrell, I Bahar, S Shapiro, D Angus, and A Kaynar, Impact of pro-domain stability of matrix metalloproteinase-8 on the outcome of sepsis, Crit Care. 2011; 15(Suppl 1): P278.